Illumina-based RNA-Seq of Peperomia dahlstedtii: mature leaf tissue - SRA

SRX15484629: Illumina-based RNA-Seq of Peperomia dahlstedtii: mature leaf tissue
1 ILLUMINA (Illumina HiSeq 4000) run: 27.7M spots, 1.4G bases, 462.9Mb downloads

Design: Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit with IDT for Illumina Unique Dual Index adapters following manufacturer's recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts and the pool quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit. The pool was loaded onto one (1) lane of an Illumina HiSeq 4000 flow cell and sequencing was performed in a 1x50bp single read format using HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.7 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.

Submitted by: Michigan State University

Study: Assembly of the Peperomia dahlstedtii genome and Peperomia pellucida genome-guided transcriptome

PRJNA843057 • SRP377436 • All experiments • All runs

show Abstracthide Abstract

The generation of the first genome assembly for Peperomia - Peperomia dahlstedtii, in addition to a reference genome-guided transcriptome assembly for Peperomia pellucida.

Sample:

SAMN28699920 • SRS13200331 • All experiments • All runs

Organism: Peperomia dahlstedtii

Library:

Name: 10-dahl_A

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 27.7M spots, 1.4G bases, 462.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR19431301	27,732,410	1.4G	462.9Mb	2023-06-01

ID:: 22053937

SRA

Sequence Read Archive

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